GSM7471637: Trichoplax sp. H2 ATAC; Trichoplax sp. H2; ATAC-seq - SRA

SRX20648978: GSM7471637: Trichoplax sp. H2 ATAC; Trichoplax sp. H2; ATAC-seq
4 ILLUMINA (NextSeq 500) runs: 129.1M spots, 10.8G bases, 4.2Gb downloads

External Id: GSM7471637_r1

Submitted by: Sebe-Pedros lab, Systems Biology, Centre for Genomic Regulation

Study: Stepwise emergence of the neuronal gene expression program in early animal evolution

PRJNA982113 • SRP442048 • All experiments • All runs

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We performed single-cell transcriptome analysis (using 10x) of four placozoan species (Trichoplax adhaerens, Trichoplax sp. H2, Cladtertia collaboinventa, Hoilungia hongkongensis). Additionally we performed bulk ATAC-seq experiments and anti-H3K4me2/anti-H3K4me3 ChIP-seq experiments the same species. Overall design: Adult specimens were fixed, dissociated and pooled before sorting single-cells for scRNAseq. Some samples were mixed (cross-species or cross-treatments) and processed together. Instructions for demultiplexing cells are provided. Please note that the processed data for the scRNA-seq samples are generated from multiple samples (as indicated in the corresponding sample descriptionfield) and thus is linked to the Series records.

Sample: Trichoplax sp. H2 ATAC

SAMN35687519 • SRS17947106 • All experiments • All runs

Organism: Trichoplax sp. H2

Library:

Name: GSM7471637

Instrument: NextSeq 500

Strategy: ATAC-seq

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: ATAC-seq was performed using the Omni-ATAC protocol (10.1038/nmeth.4396) and using custom Tn5 enzyme Adult specimens were fixed, dissociated and pooled before sorting single-cells for scRNAseq. The same procedure was followed for chromatin profiling experiments, except that cells were used fresh for ATAC-seq and fixed with 1% formaldehyde (10 min) for ChIP-seq

Runs: 4 runs, 129.1M spots, 10.8G bases, 4.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR24886397	33,684,985	2.8G	1.1Gb	2023-09-21
SRR24886398	28,101,529	2.4G	946.5Mb	2023-09-21
SRR24886399	32,576,454	2.7G	1.1Gb	2023-09-21
SRR24886400	34,704,235	2.9G	1.1Gb	2023-09-21

ID:: 28078215

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